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Specifically, IRE enhances systemic antitumor T-cell activation while downregulating immunosuppressive mechanisms in localized disease, promoting tumor antigen-specific expansion of tissue-resident memory CD8 + T cells (TRM) for durable immune surveillance ( In vitro and magnetic resonance imaging (MRI) assessments further validate IRE’s ability to achieve sustained tumor regression ( 3.4 Melanoma PEFs represent a highly suitable therapeutic approach for melanoma, primarily because these tumors are typically located in superficial layers, allowing for direct percutaneous minimally invasive intervention that yields clinically significant outcomes