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On the basis of insights gained from our previous model of HSV-1 gB, which was determined using a stabilizing helix breaker mutation (H516P) in the central helix of domain III (DIII) 16 , we devised mutations that use different means of stabilization 29 (Fig
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After differentiation with 1 μM RA, NE-4C nerve cells were incubated with a Fluo-4 AM loading buffer (100 μL/well, DOJINDO Laboratories) for 1 h at 37 °C in the dark
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